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JoVE Journal
Immunology and Infection

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In vitro Uncoating of HIV-1 Cores

In vitro Uncoating of HIV-1 Cores

Article DOI: 10.3791/3384
November 8th, 2011


Summary November 8th, 2011

Uncoating is an essential step in the early phase of the HIV-1 life cycle and is defined as the disassembly of the capsid shell and the release of the viral ribonucleoprotein complex (vRNP). Here, we demonstrate techniques for isolating intact cores from HIV-1 virions and for quantifying their uncoating in vitro.

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