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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
DOI:

07:10 min

July 29, 2016

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Chapters

  • 00:05Title
  • 00:40Tumor Dissociation
  • 02:06Magnetic Cell Separation Mouse Cell Depletion
  • 04:01Representative Results: Downstream Mouse Cell-free Human Tumor Cell Analyses
  • 05:58Conclusion

Summary

Automatic Translation

Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.

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