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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
JoVE Journal
Medicine
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JoVE Journal Medicine
Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

DOI:
10.3791/54259-v

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07:10 min

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July 29, 2016

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, , , , , , , ,
1R&D Reagents,Miltenyi Biotec GmbH, 2In vivo Tumorbiology,Oncotest GmbH

Chapters

  • 00:05Title
  • 00:40Tumor Dissociation
  • 02:06Magnetic Cell Separation Mouse Cell Depletion
  • 04:01Representative Results: Downstream Mouse Cell-free Human Tumor Cell Analyses
  • 05:58Conclusion

Summary

Automatic Translation

Automatic Translation

Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.

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