Manduca Sexta

Manduca sexta, the tobacco hornworm moth, is a well-established model organism for studying animal behavior and nervous-system function. Its behavioral responses arise from the integration of olfactory, gustatory, and mechanosensory cues, which guide feeding, locomotion, mating, and defensive actions through coordinated neural and hormonal signaling. Researchers use Manduca sexta to investigate sensory processing, decision-making, learning, and interactions between herbivorous insects and their host plants. Because its anatomy, physiology, and behavior can be examined across developmental stages, this species provides accessible experimental insights into how neural circuits generate adaptive behavior.

Manduca Sexta - Related Videos

Research

JoVE Journal - Immunology and Infection

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

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Cited by 10 •

2012

The method described here utilizes direct injection of entomopathogenic bacteria into the hemocoel of Manduca sexta insect larvae. M. sexta is a commercially available and well-studied insect. Thus, this method represents a simple approach to analyzing host-bacterial interactions from the perspective of one or both partners.

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

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Cited by 4 •

2019

The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.

Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity Cycle

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2024

Here, we detail a simple, fast, and reliable multiparametric flow cytometry- and tumor-on-a-chip-assisted protocol to monitor and characterize the steps constituting the cancer-immunity cycle. Indeed, a thorough understanding of the interplays between cancer and immune cells provides critical insights to outsmart tumors and guide clinical care.

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