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Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
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Chapters
- 00:05Title
- 01:06Insect Egg Sterilization and Rearing
- 04:06Preparation of Bacteria for Injection
- 04:44Injection of 4th Instar Larvae
- 07:46Results: Representative Insect Mortality Assay
- 08:40Conclusion
The method described here utilizes direct injection of entomopathogenic bacteria into the hemocoel of Manduca sexta insect larvae. M. sexta is a commercially available and well-studied insect. Thus, this method represents a simple approach to analyzing host-bacterial interactions from the perspective of one or both partners.
Tags
Manduca SextaTobacco HornwormAgricultural PestSolanaceous PlantsEntomopathogenic BacteriaInsect Immune SystemGenome SequenceModel OrganismHost-microbe InteractionsPathogenesisLarvae InjectionVirulence CharacteristicsBacterial SpeciesMutantsTime To Insect DeathXenorhabdus SpeciesPhotorhabdus SpeciesNematode VectorsInsect Hemolymph
