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Immunology and Infection

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JoVE Journal Immunology and Infection
Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
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Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

Article DOI: 10.3791/4295
December 11th, 2012 •
1, 1
1Department of Bacteriology, University of Wisconsin-Madison

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December 11th, 2012

The method described here utilizes direct injection of entomopathogenic bacteria into the hemocoel of Manduca sexta insect larvae. M. sexta is a commercially available and well-studied insect. Thus, this method represents a simple approach to analyzing host-bacterial interactions from the perspective of one or both partners.

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