Cardiac Metabolism Monitoring
Cardiac Metabolism Monitoring - Related Videos
15:45
Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice
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Cited by 11 •
2013
We describe the protocol to perform a cardiac stress test induced by dobutamine and monitored by cardiac catheterization in normal mice. Also we show its application to unmask subclinical cardiac disease in high fat diet-induced obese mice.
01:27
Isothermal Microcalorimetry to Monitor Metabolic Activity in Bacteria
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2025
Take serial dilutions of a bacterial culture and add aliquots into calorimetric vials containing growth medium.Load the sealed vials into the multi-well plate of an isothermal microcalorimeter to measure metabolic heat production.The device is held at a constant temperature to ensure the detected heat originates solely from bacterial metabolism.As bacteria metabolize nutrients and proliferate, they release heat that sensors detect in real time.In parallel, incubate identical vials in an...
08:51
Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts
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Cited by 3 •
2019
Here we introduce a method for using an intra-ventricle optical catheter in perfused hearts to perform absorbance spectroscopy across the heart wall. The data obtained provides robust information on tissue oxygen tension as well as substrate utilization and membrane potential simultaneously with cardiac performance measures in this ubiquitous preparation.
04:24
A Novel Digital Platform for a Monitored Home-based Cardiac Rehabilitation Program
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Cited by 12 •
2019
The aim is to promote a new approach to cardiac rehabilitation (CR), using a unique remote patient monitoring system that will enable healthcare providers to monitor CR patients at home at low cost, with the intention of making CR services more accessible and improving compliance. The study is currently underway.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
FAQs
It combines measurements of substrate utilization, oxygen consumption, glucose and fatty acid oxidation, and ATP production. Examining these variables together can indicate whether cardiac tissue is changing its preferred fuel or altering energy generation. This integrated view helps relate metabolic shifts to myocardial function rather than interpreting a single metabolic measurement in isolation.
Oxygen consumption provides a physiological measure that can be compared with energy-related outcomes such as ATP production and myocardial workload. This comparison helps researchers assess how effectively heart tissue converts available oxygen and substrates into usable energy. Changes may reveal altered metabolic performance during increased workload, ischemia, hypoxia, or other experimental conditions.
Ischemia and hypoxia can change substrate utilization, oxygen consumption, glucose and fatty acid oxidation, and ATP production. Monitoring these variables shows how cardiomyocytes respond when oxygen availability or tissue conditions are disrupted. In bioengineering studies, such measurements help evaluate whether engineered environments reproduce clinically relevant metabolic stress and how cells adapt to it.
Measurements of glucose and fatty acid oxidation provide complementary information about which substrates contribute to cardiac energy production. Comparing these pathways can identify metabolic remodeling and reveal shifts associated with workload, disease-related conditions, or engineered tissue environments. The resulting profile is more informative than assessing total energy production without examining the substrates that support it.
Researchers can integrate physiological measurements, imaging, and biochemical assays, selecting the approach according to the metabolic variable and experimental model. These tools can assess oxygen consumption, substrate oxidation, ATP-related changes, or broader responses in myocardial tissue. Combining methods allows metabolic findings to be interpreted alongside functional responses in cardiomyocytes or engineered cardiac tissues.
In engineered tissue environments, metabolic measurements help determine how cardiomyocytes respond to controlled changes in workload, oxygen conditions, or experimental treatments. The data can guide the design and evaluation of biosensors and organ-on-chip platforms. They also help establish whether an engineered model captures relevant aspects of myocardial energy use and metabolic remodeling.
The approach is useful when investigators need to examine impaired myocardial metabolism, metabolic remodeling, or altered cardiac energy efficiency. It can be applied while studying responses to ischemia, hypoxia, drugs, or changing workload. Results may support evaluation of therapeutic strategies that target myocardial metabolism and provide metabolic context for changes in heart tissue function.
Cardiac metabolism data can connect cellular energy pathways with tissue-level performance and experimental conditions. Researchers may use the results to characterize cardiomyocyte responses, assess engineered tissue environments, and evaluate metabolic effects of drugs or therapeutic interventions. These outcomes also inform the development of biosensors, organ-on-chip systems, and platforms designed to study cardiac energy efficiency.