Drug Gradient Generation
Drug Gradient Generation - Related Videos
11:05
A Gradient-generating Microfluidic Device for Cell Biology
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Cited by 14 •
2007
We describe a protocol for the microfabrication of the gradient-generating microfluidic device that can generate spatial and temporal gradients in well-defined microenvironment. In this approach, the gradient-generating microfluidic device can be used to study directed cell migration, embryogenesis, wound healing, and cancer metastasis.
05:10
Drug Repurposing Hypothesis Generation Using the "RE:fine Drugs" System
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Cited by 4 •
2016
Here we describe a protocol using the web-based drug repurposing hypothesis generation tool: "RE:fine Drugs." This protocol can be modified to a user's preferences at the level of the query type (gene, drug or disease) and/or the range of available advanced options.
10:24
Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation
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Cited by 3 •
2019
We present a microfluidic cancer-on-chip model, the "Evolution Accelerator" technology, which provides a controllable platform for long-term real-time quantitative studies of cancer dynamics within well-defined environmental conditions at the single-cell level. This technology is expected to work as an in vitro model for fundamental research or pre-clinical drug development.
04:30
A Technique to Generate and Deliver Drug Aerosols Directly to Mouse Lungs
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2025
This video demonstrates the generation of drug aerosols and the targeted nose-only delivery of these aerosols to mice using an inhalation unit equipped with an aerosol generator. The mouse is restrained in a nose-only restrainer and connected to the inhalation unit, which delivers the drug directly into the mouse's nasal cavity. Subsequently, the drug passes through the trachea and enters the lungs.
08:42
Density Gradient Ultracentrifugation
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2023
Density gradient ultracentrifugation is a common technique used to isolate and purify biomolecules and cell structures. This technique exploits the fact that, in suspension, particles that are more dense than the solvent will sediment, while those that are less dense will float. A high-speed ultracentrifuge is used to accelerate this process in order to separate biomolecules within a density gradient, which can be established by layering liquids of decreasing density in a centrifuge tube. The...
FAQs
These transport processes determine how quickly drug molecules move and how concentration changes across space or time. Diffusion spreads drug through concentration-driven movement, while convection redistributes it through directed transport. Controlled mixing provides another way to regulate exposure. Adjusting the dominant transport process changes the resulting concentration profile, allowing researchers to create defined chemical conditions for biological testing.
Measurement shows whether the intended drug distribution has formed and provides evidence of how exposure changes across the system. Researchers can then adjust the transport conditions or mixing strategy to refine the profile. This control is important because biological responses depend on the chemical environment experienced by cells or tissues, not simply on the total amount of drug introduced.
Uniform exposure presents cells or tissues with broadly similar drug conditions, whereas a gradient creates changing concentrations across space or time. The latter can reveal how responses vary with local dose and changing exposure. This distinction helps connect molecular responses to tissue-level outcomes and more closely represents drug environments that biological systems may encounter in vivo.
A typical workflow begins by selecting a transport or mixing process, establishing conditions that produce the desired concentration change, and then measuring the resulting profile. Researchers compare the measured distribution with the intended design and adjust the system when needed. The verified gradient can then be used to expose biological samples to defined, spatially or temporally varying drug conditions.
The approach supports dose-response analysis by exposing biological systems to differing drug levels, and it enables cell migration studies in chemically varied environments. It is also useful in tissue engineering, where spatially controlled conditions can be examined, and in evaluating therapeutic delivery strategies. These applications use measured concentration changes to relate drug exposure to biological behavior.
In therapeutic delivery studies, controlled concentration changes allow researchers to examine how delivery strategies influence drug exposure across a biological system. Comparing responses under defined gradients can reveal relationships between local drug conditions and tissue-level effects. This information helps evaluate delivery designs and can contribute to the development of treatments that are safer and more effective.