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Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation
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Bioengineering
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JoVE Journal Bioengineering
Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

DOI:
10.3791/60185-v

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10:24 min

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September 19, 2019

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, , , , ,
1Princeton University, 2Johns Hopkins Medical Institute

Chapters

  • 00:04Title
  • 01:09Fabrication of Metal Plate Holder and Cell Line Seeding
  • 03:05Setting up Thermal Control Unit and Gas Supply System
  • 03:50Installation of Microfluidic Device
  • 08:12Results: Growth Curves and Cell Motility Assay
  • 09:33Conclusion

Summary

Automatic Translation

Automatic Translation

We present a microfluidic cancer-on-chip model, the "Evolution Accelerator" technology, which provides a controllable platform for long-term real-time quantitative studies of cancer dynamics within well-defined environmental conditions at the single-cell level. This technology is expected to work as an in vitro model for fundamental research or pre-clinical drug development.

Tags

Keywords: Microfluidic Cancer ModelEvolution AcceleratorReal-time ObservationHeterogeneous Drug GradientsTumor-like Stress LandscapePreclinical Drug DevelopmentPressure Sealing PackagingLong-term ExperimentGas CompositionCell Line SeedingPC3-EpiPC3-EMTPDMS Devices

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