Electrophysiology Microscope Modification
Electrophysiology Microscope Modification - Related Videos
02:32
Histone Modification
0 Views •
2020
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression. Acetylation The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
10:46
Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology
0 Views •
Cited by 4 •
2015
Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.
02:25
Spreading of Chromatin Modifications
0 Views •
2020
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex. Writers The writer is an enzyme that can...
05:41
Applying Microfluidics to Electrophysiology
0 Views •
Cited by 1 •
2007
Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities. Specifically, the motivation for the ...
07:08
Detection of Histone Modifications in Plant Leaves
0 Views •
Cited by 22 •
2011
A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.
FAQs
Mechanical stability supports accurate positioning of recording electrodes or patch-clamp pipettes relative to living cells. It also helps maintain alignment during simultaneous visualization and electrical measurement, reducing inconsistencies caused by movement or shifting focus. In bioengineering experiments, this is especially important when comparing cellular responses to engineered materials, external stimuli, or therapeutic interventions.
Illumination and focus allow researchers to monitor cell morphology while recording membrane activity. Preserving these optical functions helps link changes in electrical behavior with visible cellular structure or condition. This combined observation is valuable when assessing living cells, because electrical signals can be interpreted alongside morphological responses during manipulation or exposure to experimental treatments.
Recording consistency depends on coordinated optical access, stable positioning, and reliable control of illumination and focus. The microscope must provide enough access to place electrodes or patch-clamp pipettes while maintaining a clear view of the target cell. These features support repeatable alignment and make it easier to compare electrical responses across live-cell experiments.
A typical workflow establishes the microscope as a stable imaging platform, preserves illumination and focusing, and creates access for positioning recording electrodes or patch-clamp pipettes. Researchers then align the recording tools with the selected living cells while observing morphology and membrane activity in real time. This arrangement supports coordinated manipulation, recording, and visual monitoring.
The modified setup can support live-cell models, organoids, and tissue constructs when researchers need optical monitoring during electrical recording. Its value extends beyond isolated cell observation by helping relate signals to structure in more complex engineered or biological systems. This makes the approach relevant to bioengineering studies of organized tissues and cellular interfaces.
In bioengineering, the setup helps researchers examine how cells respond to engineered materials, stimuli, or therapeutic interventions while recording electrical activity. Applications include neural interfaces, cardiac electrophysiology, and bioelectronic devices. By combining signal measurement with real-time visualization, the system can reveal relationships among cellular structure, function, and the behavior of engineered interventions.