Orthogonal Click Chemistries
Orthogonal Click Chemistries - Related Videos
01:26
Orthogonal Trajectories
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2026
Orthogonal trajectories describe the geometric relationship between two families of curves that intersect each other at right angles. One illustrative case involves a family of parabolas that open sideways along the x-axis. These curves share a common shape but differ by a scaling parameter, resulting in a set of curves that all pass through the origin and widen at different rates.Determining Orthogonal TrajectoriesTo identify the orthogonal trajectories for these parabolas, the first step...
10:32
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
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Cited by 7 •
2012
A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.
06:46
Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
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Cited by 11 •
2011
A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.
08:42
Coordination Chemistry Complexes
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2023
Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center,...
08:43
Calibration Procedures for Orthogonal Superposition Rheology
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Cited by 4 •
2020
We present a detailed calibration protocol for a commercial orthogonal superposition rheology technique using Newtonian fluids including end-effect correction factor determination methods and recommendations for best practices to reduce experimental error.
FAQs
The central design principle is chemical discrimination: each reaction recognizes its complementary handle while ignoring the other reaction partners. This mutual compatibility lets researchers assign different labels or conjugates to distinct targets in one sample. Because the reactions form covalent bonds, the resulting modifications remain chemically attached for subsequent analysis and material construction.
The reaction environment helps preserve selectivity. Orthogonal click chemistries are designed to proceed rapidly under mild, often aqueous conditions that are compatible with biological samples, while avoiding reaction with native biomolecules. This combination reduces unwanted modification and makes the chemistry suitable for labeling or conjugating components in bioengineering systems.
Orthogonality enables multiplexing because one reaction can proceed without interfering with the handles assigned to another reaction. Researchers can therefore distinguish several molecular targets or components within the same sample, improving control over which species receives each label or conjugate. This separation is especially valuable when spatial or temporal resolution matters.
A practical design workflow begins by selecting complementary chemical handles for each intended labeling or conjugation event. Researchers then combine the matched partners under mild, often aqueous conditions, while preserving the other handles for separate reactions. This multiplexed strategy supports controlled assembly of several modifications in one sample without cross-reactivity among the planned reactions.
In multiplexed imaging, different chemical handles can connect distinct labels to selected molecular components, allowing those components to be tracked separately in a shared sample. The same selective strategy supports site-specific protein modification, in which a defined conjugate is attached to a chosen protein. These uses improve molecular discrimination during bioengineering studies.
Beyond labeling, these reactions provide a way to build and functionalize engineered materials. They can support biomolecule immobilization, hydrogel fabrication, and targeted delivery by connecting selected molecular components through covalent bonds. In each case, the modular handle-and-partner design helps researchers combine multiple functions while retaining control over where and when molecular interactions are introduced.