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Journal / Biology / Detection of Histone Modifications in Plant Leaves
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Detection of Histone Modifications in Plant Leaves
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Detection of Histone Modifications in Plant Leaves

Article DOI: 10.3791/3096 • 07:09 min • September 23rd, 2011
September 23rd, 2011 •
1,2, 3, 2
1Department of Botany, RWTH Aachen University, 2Department of Plant Physiology, RWTH Aachen University, 3Department of Botany, Leibniz University

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Summary
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A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.

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Histone Modifications Plant Leaves Chromatin Structure Gene Expression Regulation Chromatin Remodeling DNA Methylation Covalent Modifications Histones H3 And H4 Lysine Methylation Arginine Methylation Lysine Acetylation Serine Phosphorylation Gene Activation Gene Repression Primed State Of Gene Chromatin Immunoprecipitation (ChIP) Modification-specific Antibodies Real-time Quantitative PCR C4 Photosynthesis In Maize Systemic Immunity In Arabidopsis
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