Ex Vivo Flow Model
Ex Vivo Flow Model - Related Videos
10:24
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
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Cited by 13 •
2015
To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
07:16
In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry
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Cited by 15 •
2015
The malaria parasite invades and replicates within red blood cells. The accurate assessment of merozoite invasion and parasitemia is therefore crucial in assessing the course of malaria infection. Here we describe a flow cytometry based protocol for the measurement of these parameters in a mouse model of malaria.
08:09
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
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Cited by 24 •
2015
This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.
12:31
An Ex vivo Model to Study Hormone Action in the Human Breast
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Cited by 8 •
2015
We have developed a novel ex vivo model to study hormone action in the human breast. It is based on tissue microstructures isolated from surgical breast tissue specimens which preserve tissue architecture, intercellular interactions, and paracrine signaling.
FAQs
Flow regulation is important because it converts fluid movement into defined mechanical inputs, especially shear stress and cyclic loading. By adjusting flow rate, pressure, and fluid composition, researchers can separate responses to physical forces from responses to biochemical conditions. This control helps reveal how tissues or resident cells react under physiological or disease-relevant environments rather than under static culture conditions.
Preserving native architecture allows researchers to examine responses within the structural context of an isolated tissue, organ, or biological structure. That context can retain relationships that simplified in vitro assays do not reproduce, while the external circuit still permits controlled experimental conditions. The resulting system supports more biologically relevant analysis without requiring immediate reliance on whole-animal experiments.
A static in vitro assay offers experimental simplicity but may not reproduce fluid movement, mechanical forces, or native tissue organization. A whole-animal study includes systemic biology but provides less direct control over local flow and biochemical conditions. An ex vivo flow model occupies an intermediate position, combining preserved biological structure with adjustable physical and chemical inputs.
Fluid composition provides a biochemical dimension that complements mechanical stimulation from flow and pressure. Changing the composition can help researchers examine how tissues respond when biochemical conditions vary while the surrounding structure remains isolated and the circuit remains controllable. This combined control is useful for separating mechanical effects from biochemical influences in tissue and cell-response studies.
The workflow centers on placing an isolated tissue, organ, or biological structure within a fluid circuit, then using a pump or pressure-driven system to regulate movement through or around it. Researchers establish the desired flow rate, pressure, and fluid composition before examining biological responses. These controls make the setup adaptable to physiological or disease-relevant conditions.
This approach enables examination of how cells and tissues respond to controlled mechanical and biochemical conditions. Researchers can investigate effects associated with shear stress, cyclic loading, fluid composition, and their combined influence while maintaining aspects of native structure. The resulting observations can inform studies of tissue behavior, biomaterial interactions, drug delivery, and medical-device performance.
Bioengineers may select this model when they need more biological context than a simplified in vitro assay provides but more experimental control than a whole-animal study allows. Supported applications include vascular biology, tissue engineering, biomaterials, drug delivery, and evaluation of medical-device performance. Its value comes from testing controlled conditions while retaining aspects of isolated native tissue organization.
The system allows engineered tissues, biomaterials, drug-delivery strategies, and medical devices to be examined under controlled fluid movement and related mechanical or biochemical conditions. In bioengineering, those tests can connect material or device behavior with tissue responses. This makes the model a practical bridge between simplified laboratory assays and more complex whole-animal evaluation.