Highly Inclined Swept Tile Microscopy
Highly Inclined Swept Tile Microscopy - Related Videos
08:13
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
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Cited by 2 •
2019
A detailed instruction is described on how to build a highly inclined swept tile (HIST) microscope and its usage for single-molecule imaging.
01:25
Inclination of a Line
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2025
The inclination of a line describes its angle of tilt with respect to the horizontal axis. While a line itself is an abstract object with no thickness, its orientation on the Cartesian plane is determined by its slope, which reflects how steeply it rises or falls. The inclination angle, always measured counterclockwise from the positive x-axis, varies between zero and π radians for nonhorizontal lines. This angle directly relates to the slope, providing a geometric interpretation of the line's...
10:07
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
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2014
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
13:29
Jet Impinging on an Inclined Plate
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The goal of this experiment is to demonstrate how a fluid flow exerts forces on structures by conversion of dynamic pressure into static pressure. To this end, we will make a plane jet impinge on a flat plate and will measure the resulting pressure distribution along the plate. The resultant force will be estimated by integrating the product between the...
01:11
Work Done Over an Inclined Plane
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2023
The center-of-mass framework helps to easily describe the work done on rigid bodies. Since the internal forces in a rigid body do no work, they can be ignored, and the external forces can be considered in the work-energy theorem. The work done by gravity to move a rigid body, or the work done by an opposing force to move a rigid body against gravity, can be calculated using the center-of-mass framework. It is the line integral of the force of gravity over the path, considered positive if...
FAQs
The steeply angled excitation beam concentrates illumination within a limited region of the specimen rather than broadly exciting surrounding material. As the beam is swept through the sample, this geometry produces optical sectioning and limits out-of-focus fluorescence. The resulting images can therefore preserve high-resolution structural information while covering specimens whose organization extends beyond a single focal view.
Sweeping moves the thin excitation beam through different positions in the sample, allowing successive optical sections to be recorded across the region of interest. Because excitation is confined during each position, fluorescence from outside the imaged section is limited. This supports detailed visualization of cellular and tissue structures without relying on one undifferentiated view of the specimen.
Tiling separates a large specimen into adjacent image regions that can be acquired at high resolution and later assembled. This preserves fine spatial detail within each tile while extending the overall field of view beyond what a single image can capture. The assembled result helps connect local cellular organization with larger tissue-scale architecture.
The workflow begins by directing a thin excitation beam through the specimen at a steep angle and sweeping it to obtain optically sectioned image data. Adjacent regions are then captured as separate tiles. These tiles are assembled into a larger image, creating a view that combines localized high-resolution information with broader spatial coverage.
Its stated applications include cells, tissues, biomaterials, and engineered constructs. This range makes the approach useful when investigators need to examine fine cellular features while also considering how those features are distributed across a larger engineered or biological structure. The method can therefore connect microscopic organization with the architecture of bioengineering specimens.
By linking cellular organization to tissue-scale structure, the technique can provide spatial context for changes occurring in developing, diseased, or regenerating specimens. In regenerative engineering, that context may help investigators examine engineered constructs alongside their cellular and tissue organization. Its value lies in combining detailed views with coverage broad enough to evaluate larger biological patterns.