Image-guided Laser Degradation
Image-guided Laser Degradation - Related Videos
10:53
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Cited by 10 •
2017
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
06:40
Laser-guided Neuronal Tracing In Brain Explants
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2015
We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.
09:29
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
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Cited by 3 •
2017
We describe the use of laser capture microdissection to obtain samples of distinct cell populations from different brain regions for gene and microRNA analysis. This technique allows the study of differential effects of traumatic brain injury in specific regions of the rat brain.
02:58
Regulated Protein Degradation
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2020
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells. Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
02:11
Proteins: From Genes to Degradation
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2020
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation. Transcription is the synthesis of RNA molecules by RNA...
FAQs
Real-time imaging provides visual feedback about the target's location and the laser's position relative to surrounding regions. This allows the operator or system to adjust where energy is delivered and regulate exposure and dose while the process proceeds. The result is tighter spatial control, which is especially important when the selected tissue or material lies near structures that should remain intact.
The absorbed laser light can produce different localized effects, including heating, photochemical change, or mechanical disruption. In Image-guided Laser Degradation, the relevant outcome depends on how the delivered energy interacts with the selected tissue or engineered material. Controlling exposure and dose helps tailor the degradation process to the target and limits unintended effects in adjacent regions.
Spatial selectivity allows degradation to be confined to a chosen region rather than distributed broadly across the sample. Combined with visual feedback, this precision supports controlled tissue remodeling, localized microsurgery, and engineered-material processing. It also helps investigators alter a defined structure while preserving neighboring regions, making it possible to examine how localized changes influence biological structure and function.
A typical workflow begins by using imaging to identify the tissue or engineered material selected for removal or breakdown. The imaging information then guides laser positioning, while exposure and dose are controlled for the target region. Absorbed light produces the intended localized effect, and visual feedback supports adjustment of the process as degradation proceeds.
The technique supports several distinct bioengineering uses. In microsurgery, it can enable localized removal; in tissue remodeling, it can modify selected biological regions; and in device fabrication, it can break down engineered materials with spatial control. Researchers can also use these targeted changes to investigate structure-function relationships in living systems.
By selectively degrading a defined tissue region while limiting effects elsewhere, the method can create controlled changes in biological structure. Researchers can then examine how those changes relate to function, supporting studies of structure-function relationships. Its combination of visual feedback and localized energy delivery also contributes to minimally invasive therapeutic and engineering strategies.