Journal
/
Neuroscience
/
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

DOI:
10.3791/56134-v

•

09:29 min

•

September 11, 2017

•

, , ,
1Department of Anesthesiology,University of Texas Medical Branch

Chapters

  • 00:05Title
  • 00:59Region Identification and Sectioning
  • 04:01Staining Protocol
  • 04:50Stereotactic Atlas Guided Laser Capture Microdissection with the LCM System
  • 07:36Results: LCM of Traumatic Brain Injury affected Brain Regions
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of laser capture microdissection to obtain samples of distinct cell populations from different brain regions for gene and microRNA analysis. This technique allows the study of differential effects of traumatic brain injury in specific regions of the rat brain.

Tags

Keywords: Stereotactic AtlasLaser Capture MicrodissectionTraumatic Brain InjuryFrontal CortexHippocampusMolecular AnalysisTranscriptional AnalysisCryostatOptimal Cutting Temperature MediumBrain RegionsCell Types

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56134?article_type=v

Laser Capture Microdissection of Drosophila Peripheral Neurons
check_url/56134?article_type=v

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice
check_url/56134?article_type=v

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
check_url/56134?article_type=v

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury
check_url/56134?article_type=v

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
check_url/56134?article_type=v

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
check_url/56134?article_type=v

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
check_url/56134?article_type=v

Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
check_url/56134?article_type=v

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila
check_url/56134?article_type=v

A Novel In Vitro Model of Blast Traumatic Brain Injury

Read Article