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Laser-guided Neuronal Tracing In Brain Explants

Laser-guided Neuronal Tracing In Brain Explants

Article DOI: 10.3791/53333
November 25th, 2015


Summary November 25th, 2015

We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

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