Laser Irradiation
Laser Irradiation - Related Videos
08:48
Selective Area Modification of Silicon Surface Wettability by Pulsed UV Laser Irradiation in Liquid Environment
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Cited by 1 •
2015
We report on a process of in situ alteration of HF treated Si (001) surface into a hydrophilic or hydrophobic state by irradiating samples in microfluidic chambers filled with H2O2/H2O solution (0.01%-0.5%) or methanol solutions using pulsed UV laser of a relative low pulse fluence.
08:18
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
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Cited by 30 •
2017
Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.
07:11
Laser Micro-Irradiation to Study DNA Recruitment During S Phase
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Cited by 6 •
2021
This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser micro-irradiation.
06:40
Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments
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Cited by 5 •
2021
A protocol is presented for automated irradiation of thin gold foils with high intensity laser pulses. The protocol includes a step-by-step description of the micromachining target fabrication process and a detailed guide for how targets are brought to the laser's focus at a rate of 0.2 Hz.
04:15
A Mouse Model of Fatigue Induced by Peripheral Irradiation
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Cited by 18 •
2017
We describe a method using targeted peripheral irradiation to induce fatigue-like behavior in mice. The selected non-lethal irradiation dose leads to a week-long reduction in voluntary wheel-running activity.
FAQs
Wavelength determines which chromophores or other absorbing components can capture photons, so it helps shape where optical energy is deposited and which response is favored. In a bioengineering experiment, researchers can use wavelength as a primary control for targeting a material, cell, or tissue component while limiting unintended effects elsewhere. Its importance is greatest when spatial selectivity matters.
These response modes describe different consequences of photon absorption. Photothermal effects convert optical energy into heat, photochemical effects produce chemically mediated changes, and photobiomodulatory effects generate signals that influence cellular behavior. Distinguishing among them helps researchers interpret whether an outcome reflects heating, chemical activity, or biological modulation, which supports more deliberate design of bioengineering experiments.
The outcome depends on the combined irradiation dose, power, duration, exposure geometry, and properties of the material or tissue. Absorption characteristics influence how energy is distributed, while geometry affects localization. Adjusting these variables allows researchers to distinguish localized processing from broader effects and to improve the consistency of cellular, tissue, or biomaterial responses.
A reproducible protocol should identify the wavelength, power, exposure duration, and geometry, together with the material, cell, or tissue receiving the light. These parameters establish the intended optical dose and response range. Careful specification is especially important when comparing samples, linking irradiation conditions to biological outcomes, or optimizing fabrication accuracy.
In bioengineering workflows, controlled exposure can support localized heating, ablation, and the creation of defined surface patterns. Researchers adjust irradiation conditions to process selected regions rather than treating an entire material uniformly. These capabilities can improve fabrication accuracy and provide structured interfaces for investigating how cells interact with engineered materials.
Researchers can use localized exposure to investigate or modulate cellular behavior and to examine how cells respond to engineered material surfaces. The approach also contributes to minimally invasive therapeutic and regenerative strategies by enabling spatially controlled optical effects. Results can reveal how irradiation conditions, tissue properties, and cell-material interactions relate to biological outcomes.