/
/
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
This content is Free Access.
Chapters
- 00:05Title
- 00:31Laser Micro-irradiation
- 04:02Live Cell Image Analysis of the Recruitment of Fluorescent Proteins to Micro-irradiated Sites
- 05:54Results: Laser Micro-irradaition is a Tool for Characterizing DNA Damage and Repair
- 07:13Conclusion
Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.
