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Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

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08:18 min

September 05, 2017

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08:18 min
September 05, 2017

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Summary

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Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.

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