Macroporous Scaffolds
Macroporous Scaffolds - Related Videos
07:32
Interlinked Macroporous 3D Scaffolds from Microgel Rods
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2022
Microgel rods with complementary reactive groups are produced via microfluidics with the ability to interlink in aqueous solution. The anisometric microgels jam and interlink into stable constructs with larger pores compared to spherical-based systems. Microgels modified with GRGDS-PC form macroporous 3D constructs that can be used for cell culture.
11:16
Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
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Cited by 8 •
2022
Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.
07:37
Preparation of Macroporous Epitaxial Quartz Films on Silicon by Chemical Solution Deposition
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Cited by 7 •
2015
A protocol is presented for the preparation of piezoelectric macroporous epitaxial films of quartz on silicon by solution chemistry using dip-coating and thermal treatments in air.
10:08
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
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Cited by 38 •
2009
The process of electrospinning polymers for tissue engineering and cell culture is addressed in this article. Specifically, the electrospinning of photoreactive macromers with additional processing capabilities of photopatterning and multi-polymer electrospinning is described.
10:27
Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
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Cited by 12 •
2015
A procedure for the preparation of porous hybrid separation media composed of a macroporous polymer monolith internally coated by a high surface area microporous coordination polymer is presented.
FAQs
Interconnected pores are important because pore size alone does not determine whether cells and fluids can move through a scaffold. Connectivity creates continuous pathways for nutrient and waste exchange and can permit cells to migrate beyond the outer surface. In tissue engineering, this architecture helps distribute biological activity through the construct rather than concentrating it at its edges.
Macroporous scaffold design requires balancing several properties rather than maximizing porosity. Material selection and fabrication must coordinate pore size and connectivity with mechanical strength and degradation behavior. A structure that supports temporary loading while native extracellular matrix develops may need a different balance from one intended primarily to promote transport, so architecture is matched to the intended tissue-engineering role.
Architecture influences biological performance through more than simple space for cells. Interconnected pores support attachment and migration, while suitable pathways enable fluid exchange and, in some applications, vascular ingrowth. These effects make pore organization a functional design variable: it affects how cells populate the construct, how developing tissue is organized, and how the scaffold transitions toward newly formed tissue.
Design begins by selecting a biomaterial and fabrication approach, then specifying the pore architecture needed for the application. Researchers adjust pore size and connectivity while considering mechanical strength and degradation behavior. The resulting scaffold is intended to provide temporary structural support and permit transport and cell migration, after which its performance can be considered in relation to tissue formation.
Bioengineers use these scaffolds when new tissue needs both a three-dimensional framework and access to transported nutrients and waste removal. Their roles include guiding tissue organization, supporting regenerative medicine strategies, and creating in vitro models. In each case, the design connects the scaffold's temporary structural function with the biological process of extracellular matrix development.
Assessment focuses on whether the architecture supports the intended biological and structural functions. Relevant outcomes include cell attachment, migration throughout the construct, tissue formation, fluid exchange, and maintenance of temporary support as native extracellular matrix develops. When vascular ingrowth is part of the application, the interconnected pore arrangement is also relevant to that regenerative objective.