Multilayered Epithelium
Multilayered Epithelium - Related Videos
05:11
Inkjet-printed Polyvinyl Alcohol Multilayers
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Cited by 4 •
2017
An inkjet printer was used to manufacture polyvinyl alcohol multilayers. Polyvinyl alcohol water-based ink was formulated, and the main physical properties were investigated.
10:18
Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
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Cited by 26 •
2017
Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.
03:38
Isolation of Circumvallate Papillae (CVP) Epithelium from Mouse Model: An Enzymatic Procedure to Separate CVP Epithelium from Mouse Tongue
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2025
In this video, we describe the isolation of circumvallate papillae (CVP) epithelium from a murine tongue.
08:27
Multielectrode Array Recordings of the Vomeronasal Epithelium
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Cited by 14 •
2010
Multielectrode array (MEA) recordings provide a method for studying the electrical activity of large populations of neurons. Here, we present the details of a MEA preparation to record from the mouse vomeronasal epithelium while simultaneously stimulating the tissue.
08:39
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Cited by 4 •
2011
Cells play an instrumental and increasing role in research, and the discovery and development of new therapeutics. With this increasing need for greater number of cells we need more efficient and effective ways for growing and harvesting attachment dependent cells. A Multilayered flask with the right features can serve this purpose.
FAQs
Basal cells provide the renewing compartment in a bioengineered multilayered epithelium. They divide near the supporting substrate, then their descendants move outward and differentiate into specialized surface layers. This spatial progression creates tissue organization rather than a uniform cell sheet, allowing the model to reproduce how epithelial structure develops while remaining connected to its engineered support.
Cell junctions and extracellular matrix interactions help organize neighboring cells and their attachment to the supporting environment. Together with controlled access to nutrients or air, these cues influence how cells maintain layered architecture and barrier behavior. In engineered systems, adjusting these conditions is therefore central to producing a tissue model with consistent organization and interpretable responses.
As basal-cell descendants migrate outward, they do not simply add more material; they differentiate into specialized surface layers. That progression gives the tissue distinct internal and external cellular states, which is important for studying barrier function and repair. In bioengineering, reproducing this sequence helps make substitutes that better reflect organized epithelial tissue.
An engineered model begins with cells established near a supporting substrate, where basal cells can proliferate. The system then supports outward migration and differentiation while controlling exposure to nutrients or air. Researchers can assess the resulting organization and barrier behavior, then use the model to examine wound repair, disease processes, or responses to drugs and biomaterials.
Researchers apply these systems to investigate barrier function, wound repair, disease processes, and cellular responses to drugs or biomaterials. Because the model recreates layered organization under controlled conditions, changes in tissue behavior can be examined in relation to a defined experimental treatment or injury-related process. This makes it useful for mechanistic studies as well as testing.
Multilayered epithelium models are relevant to regenerative medicine because they support development of tissue substitutes for skin, airway, intestinal, and other epithelial tissues. Their value lies in combining organized cell layers with engineered support and controlled environmental exposure. The resulting constructs can provide platforms for studying replacement strategies while also serving as models for disease and treatment responses.