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Multielectrode Array Recordings of the Vomeronasal Epithelium

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Multielectrode Array Recordings of the Vomeronasal Epithelium

Multielectrode Array Recordings of the Vomeronasal Epithelium

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12,678 Views

08:27 min

March 1, 2010

DOI: 10.3791/1845-v

Hannah A. Arnson¹, Xiaoyan Fu¹, Timothy E. Holy¹

¹Anatomy and Neurobiology,Washington University School of Medicine

Multielectrode array (MEA) recordings provide a method for studying the electrical activity of large populations of neurons. Here, we present the details of a MEA preparation to record from the mouse vomeronasal epithelium while simultaneously stimulating the tissue.

Planar multi electrode arrays are used to record from the UL nasal organ. The VNO is removed from the mouse such that the neuro epithelium remains intact. First, the VNO capsule is removed from the mouse.

Then the neuro epithelium is isolated from the bony capsule, blood vessel and basal lamina. The tissue is placed in the MEA with the dendritic knob side up. Once this setup is complete, we use a robotic arm to administer liquid stimuli to the tissue.

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Multielectrode Array Recordings Vomeronasal Epithelium Neural Circuits In Vitro Studies Planar Multielectrode Array (MEA)Mouse Vomeronasal Organ (VNO)Pheromones Social Cues Sensory Neurons Receptors Robotic Liquid Handler Chemical Stimuli Sensory Responses Neuroepithelium Ligand Activity

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