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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
JoVE Journal
Biology
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JoVE Journal Biology
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
DOI:

08:39 min

December 05, 2011

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Chapters

  • 00:05Title
  • 01:00Design and Preparation of Falcon Multi-Flasks and Addition of Cell Suspensions
  • 02:19Mixing of Cells: Equilibration and Partitioning of Fluids
  • 03:23Transportation and Placement of the Multi-Flask
  • 04:12Media Exchange and Cell Harvesting
  • 05:40Recommended Working Volume in Falcon Multi-Flasks
  • 06:10Homogenous Cell-Population Generation
  • 08:25Conclusion

Summary

Automatic Translation

Cells play an instrumental and increasing role in research, and the discovery and development of new therapeutics. With this increasing need for greater number of cells we need more efficient and effective ways for growing and harvesting attachment dependent cells. A Multilayered flask with the right features can serve this purpose.

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