293t Cells
293t Cells - Related Videos
09:02
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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Cited by 1 •
2020
This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).
09:40
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
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Cited by 16 •
2007
This technique demonstrates an efficient way to prepare replication-defective retroviral stocks encoding a human oncogene, and subsequently used for induction of myeloproliferative disease in the mouse model.
09:29
Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems
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Cited by 1 •
2024
This protocol describes an extrachromosomal nonhomologous end joining (NHEJ) assay and homologous recombination (HR) assay to quantify the efficiency of NHEJ and HR in HEK-293T cells.
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Cited by 23 •
2011
Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
FAQs
The SV40 large T antigen acts as the key replication-supporting component in this system. By binding SV40 origin sequences, it allows compatible plasmids to replicate episomally. This mechanism helps sustain introduced genetic material for strong experimental expression, making plasmid design and the presence of a compatible SV40 origin important considerations.
Replication support is not universal for every plasmid. The large T antigen supports episomal replication specifically for plasmids carrying compatible SV40 origin sequences. Consequently, researchers must distinguish between the cells' ability to express an introduced construct after transfection and the additional replication support available to a compatible plasmid. That distinction helps explain differences among experimental designs.
High transfection efficiency increases the likelihood that introduced genetic material enters cells and produces detectable expression. In 293T experiments, this supports strong but transient production of the encoded gene product, which is useful when researchers need to examine gene activity during a defined experiment. The result is a practical platform for short-term molecular and cellular studies.
Episomal replication and transient expression address separate parts of an experiment. Replication of a compatible plasmid is linked to SV40 origin sequences and the large T antigen, whereas transient expression describes the strong, temporary production of an introduced gene product. Considering both features helps researchers interpret whether an outcome reflects plasmid replication support, expression capacity, or both.
To produce recombinant proteins, researchers take advantage of the cells' high transfection efficiency to introduce genetic material encoding the protein of interest. The cells then provide a setting for strong transient expression, allowing the introduced construct to generate recombinant product for molecular and cell biology studies. This application uses their expression capacity for experimental protein production.
293T cells are used to package recombinant viral vectors, extending their role beyond ordinary gene-expression experiments. Their high transfection efficiency supports introduction of genetic material used in that production setting, while strong transient expression can provide relevant introduced products during the experiment. This makes the line useful in gene-delivery and genetic-engineering research.
The platform supports studies of gene function, signaling, and cellular processes, allowing researchers to connect an introduced gene with a cellular response or molecular outcome. Its versatility enables the same general cell system to serve different experimental aims, while its reproducibility is valuable when comparing results across molecular and cell biology studies.