Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).
Senatore, A., Boone, A. N., Spafford, J. D. Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells. J. Vis. Exp. (47), e2314, doi:10.3791/2314 (2011).