3d Cell Models
3d Cell Models - Related Videos
08:31
Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma
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2025
This protocol provides a detailed description of the generation, culture, and analysis of mantle cell lymphoma in 3D printed hydrogel tumor slices.
09:17
3D Printing of Biomolecular Models for Research and Pedagogy
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Cited by 29 •
2017
Physical models of biomolecules can facilitate an understanding of their structure-function for the researcher, aid in communication between researchers, and serve as an educational tool in pedagogical endeavors. Here, we provide detailed guidance for the 3D printing of accurate models of biomolecules using fused filament fabrication desktop 3D printers.
08:08
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
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Cited by 16 •
2014
This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
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Cited by 80 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
03:53
Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model
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2023
The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.
FAQs
Their three-dimensional organization allows cells to form cell-cell and cell-matrix interactions across a spatial structure. This arrangement produces gradients of nutrients, oxygen, and signaling molecules rather than exposing every cell to the same environment. As a result, researchers can examine how location, local conditions, and interactions influence cellular behavior within a tissue-like setting.
Conventional two-dimensional cultures do not reproduce the same spatial architecture and interaction patterns found in tissues. In contrast, three-dimensional systems create organized structures and internal gradients that more closely reflect those conditions. This added physiological relevance helps investigators study tissue development, disease mechanisms, and treatment responses in an environment that bridges simple cell culture and complex organisms.
Biomaterial scaffolds provide a three-dimensional setting in which cells can organize and interact with surrounding material. Alongside aggregates, spheroids, and organoids, they offer a way to represent tissue architecture and cell-matrix relationships. Their use is especially relevant to tissue engineering and regenerative research, where spatial organization is an important part of the biological context.
Researchers can grow cells as aggregates, spheroids, or organoids, or place them within biomaterial scaffolds. These formats allow cells to develop three-dimensional organization and generate local gradients of nutrients, oxygen, and signaling molecules. The resulting systems can then support investigations of cellular behavior, tissue development, disease processes, or responses to drugs and other treatments.
They are useful when the question depends on tissue architecture, cell-cell communication, cell-matrix interactions, or spatially varying conditions. Biology studies may apply them to tissue development, disease mechanisms, cellular behavior, and responses to drugs or other treatments. Their greater physiological relevance makes them a useful intermediate approach between conventional cell culture and complex organisms.
These systems can show how cells behave within organized structures and how treatments act under gradients of nutrients, oxygen, and signaling molecules. That context supports examination of disease mechanisms and treatment responses while preserving interactions that occur within tissues. The information can therefore help connect observations from basic cell culture with biological behavior in more complex settings.