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Chapters
- 00:05Title
- 01:063D Culture of Breast Cancer Cells in Basement Membrane Matrix
- 03:58Examination of Morphogenic Features of 3D Cultures with Immunofluorescence
- 06:17Results: Representative 3D MDA-MB-231 Cell Basement Membrane Matrix Invasion Images
- 07:33Conclusion
This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.
