Adenoviral Vector Transfection
Adenoviral Vector Transfection - Related Videos
03:20
Large-Scale Production of High-Capacity Adenoviral Vectors
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2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the large-scale amplification of high-capacity adenoviral vectors in suspension-adapted human embryonic kidney cells expressing Cre recombinase for use in gene delivery applications requiring high-titer, helper-free vector preparations.
04:35
Cesium Chloride-Based Purification of Genome-Containing Adenoviral Vectors
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2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the purification of high-capacity adenoviral vectors using cesium chloride gradient ultracentrifugation, enabling the separation of genome-containing virions for applications in gene delivery, vector characterization, and preclinical therapeutic...
16:59
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
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Cited by 5 •
2012
We describe hepatic neo-islet formation in STZ (streptozotocin)-induced diabetic mice by gene transfer of Neurogenin3 (Ngn3) and Betacellulin (Btc) using helper-dependent adenoviral vector (HDAd) and the reversal of hyperglycemia. Our method takes advantages of helper-dependent adenoviral vectors with their highly efficient in vivo transduction and the long lasting gene expression.
13:17
Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
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Cited by 16 •
2016
A protocol for generation of high-capacity adenoviral vectors lacking all viral coding sequences is presented. Cloning of transgenes contained in the vector genome is based on homing endonucleases. Virus amplification in producer cells grown as adherent cells and in suspension relies on a helper virus providing viral genes in trans.
15:33
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Cited by 7 •
2013
Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.
FAQs
Cell entry follows a receptor-dependent sequence: the engineered virus first binds receptors on the target-cell surface, then enters through receptor-mediated endocytosis. After internalization, the vector delivers its DNA cargo to the nucleus. This trafficking pathway is central to the method’s ability to produce strong gene expression in appropriately targeted cells.
Because the delivered DNA generally remains episomal, it does not become part of the host genome. This supports gene expression without relying on genomic integration, while also contributing to expression that is usually transient. The distinction is especially relevant when researchers need strong expression in both dividing and nondividing cells.
Vector design can affect three important outcomes: the strength and duration of expression, the cells that receive the genetic cargo, and the immune response associated with delivery. Researchers therefore modify vector features to improve cellular targeting, regulate how long expression persists, or help control immune effects in biological studies and therapeutic research.
A typical workflow begins with selecting an engineered, replication-deficient vector carrying the desired DNA cargo and exposing appropriate target cells to it. The vector binds cell-surface receptors, enters by receptor-mediated endocytosis, and delivers the cargo to the nucleus. Researchers then assess the resulting gene expression or biological effect in the treated cells.
Researchers may select this approach for functional gene studies, protein production, vaccine development, or experimental gene therapy. Its capacity for strong expression makes it useful when investigators need to examine how a gene changes cell behavior or generate a protein, while its activity in dividing and nondividing cells broadens the range of possible target systems.
Experiments can reveal whether introducing a particular DNA cargo changes gene expression or produces a desired protein in target cells. The method can also support vaccine-related studies and experimental gene therapy research. Interpretation should account for the generally transient nature of expression, especially when evaluating effects over time or comparing different vector designs.