Agarose E3 Solution
Agarose E3 Solution - Related Videos
04:26
Agarose-Based Preparation of Human Precision-Cut Lung Slices
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2025
In this video, we describe a procedure to inflate isolated human lungs with agarose and prepare human precision-cut lung slices. The prepared ex vivo lung slices can be used for further immunologic experiments.
06:06
In Vitro Analysis of E3 Ubiquitin Ligase Function
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Cited by 8 •
2021
The present study provides detailed in vitro ubiquitylation assay protocols for the analysis of E3 ubiquitin ligase catalytic activity. Recombinant proteins were expressed using prokaryotic systems such as Escherichia coli culture.
03:55
Agarose Spin Down Assay: A Technique to Embed Organoids for Histological Analysis
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2023
In this video, we perform a spin-down assay to embed 3D prostate cancer organoids in agarose. These embedded organoids can be used for further histopathological analysis to study cancer growth and development.
02:35
DNA Agarose Gel Electrophoresis
0 Views •
2021
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol. Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA. In cloning experiments, both the insert and vector DNA...
05:55
Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
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2023
This video describes a method of preparing lungs for ex vivo live imaging to study interactions between cancer cells and stromal cells, using agarose for inflation. In the sample protocol, agarose inflation procedure is described before observing live cellular interactions.
FAQs
Cooling allows agarose polymer chains to organize into a porous network. This transition changes the preparation from a fluid that can surround and position an embryo into a gel-like matrix that holds it in place. The resulting support reduces movement during handling and observation, making embryo orientation more stable for developmental analysis.
The preparation must be fluid during embryo placement and become a supporting matrix afterward. Warming preserves flow for positioning, whereas cooling provides the stability needed for observation or manipulation. This change in physical state connects the mounting step with later imaging and experimental work, helping researchers position embryos without relying on continuous manual restraint.
The porous network physically supports the embryo while leaving surrounding E3 embryo medium available for hydration. Because the embryo remains held in position, researchers can observe morphology, movement, and experimental responses more directly. Stable positioning also improves reproducibility by reducing changes in orientation that could complicate comparisons between observations.
E3 embryo medium provides the surrounding liquid environment within the agarose preparation and helps maintain embryo hydration during manipulation. Its presence makes the matrix more suitable for handling developing zebrafish embryos than a positioning material considered alone. This combination supports workflows that require both physical stability and continued observation of developmental features.
A typical workflow uses the temperature-dependent properties of the preparation: keep it fluid while placing and positioning developing zebrafish embryos, then allow it to cool so the agarose network holds them in place. Once stabilized, the mounted embryos can undergo microscopic observation, microinjection, or other developmental biology manipulations described by the experiment.
Researchers can use this preparation when embryos must remain positioned during microscopy, microinjection, or developmental observation. It is especially relevant when experiments assess morphology, movement, or responses over an observation period, because the matrix supports consistent viewing. The same straightforward composition also makes it useful in teaching workflows as well as research.