Agilent 2100 Bioanalyzer
Agilent 2100 Bioanalyzer - Related Videos
09:15
DNA-based Fish Species Identification Protocol
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Cited by 9 •
2010
This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.
14:13
A Training Program Using an Agility Ladder for Community-Dwelling Older Adults
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Cited by 11 •
2020
The aim of this study is to present an agility training program for older people. The feasibility of this program is described, and the training protocol demonstrated by video imaging.
08:55
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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Cited by 13 •
2012
This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.
08:37
Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer
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2024
Alcohol misuse impairs alveolar macrophage (AM) immunity due to suppressed mitochondrial respiration and bioenergetics. We recently demonstrated that ethanol (EtOH) exposure increases glutamine dependency for mitochondrial respiration in AMs. Herein, methods are provided to determine the usage of glutamine for mitochondrial respiration in EtOH-treated AMs using an extracellular flux bioanalyzer.
12:04
Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
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Cited by 25 •
2009
In this video-article we present a method for the isolation and purification of Drosophila peripheral neurons using a fast magnetic bead assisted cell sorting strategy. RNA obtained from the isolated cells can be readily used for downstream applications including microarray analyses.
FAQs
Inside each disposable Lab-on-a-Chip channel, electrophoresis separates molecules according to size. Fluorescent labeling allows the separated material to be detected, and the resulting signal is represented as an electropherogram alongside numerical measurements. This combination links a physical separation step with quantitative readouts, allowing researchers to evaluate sample size, concentration, and integrity rather than relying on a single characteristic.
The disposable chip format standardizes how samples move through the analysis and reduces hands-on processing. That consistency supports comparable quality-control decisions across biological experiments. Because the channels provide a controlled microfluidic pathway for each analysis, researchers can identify unsuitable material before committing it to later work, helping improve reproducibility in downstream experimental workflows.
An electropherogram provides a record of how the detected sample material appears after electrophoretic separation. Researchers can examine this record together with numerical measurements to assess molecular size, concentration, and integrity. Using both forms of output gives quality control more context than a single number and helps reveal whether material is suitable for its intended biological experiment.
Concentration describes the amount of RNA, whereas integrity addresses the condition of that material. Measuring both prevents researchers from treating quantity as a complete measure of sample quality. This distinction is especially important before gene-expression studies or sequencing, where identifying poor-quality RNA early can guide experimental decisions and reduce the risk of using unsuitable material.
Researchers can analyze RNA before beginning gene-expression studies to obtain integrity and concentration information. These measurements help determine whether the starting material is appropriate for the planned work and can identify poor-quality samples at an early stage. Early screening supports more reproducible decisions by separating sample-quality problems from issues that arise later in the experiment.
DNA libraries and PCR products can be examined for molecular size, concentration, and integrity using the instrument’s electrophoretic and fluorescent readouts. The resulting data provide quality-control information before these materials are used in subsequent biological research. Detecting unsuitable DNA early helps researchers make informed decisions about whether a library or amplified product is ready for further work.
The system can also examine some protein samples, extending its use beyond nucleic-acid quality control. For these samples, the instrument generates analytical readouts that researchers can use to assess relevant size, concentration, and integrity information supported by the assay. This broader capability allows one standardized platform to contribute to multiple types of biological sample evaluation.