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Biology

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In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
 

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Article doi: 10.3791/3702
March 26th, 2012

Summary March 26th, 2012

This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

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