Amniotic Membrane Dissection
Amniotic Membrane Dissection - Related Videos
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Cited by 3 •
2017
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
04:21
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
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2022
The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.
07:36
Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
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Cited by 20 •
2015
Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.
10:00
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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Cited by 8 •
2019
This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
11:33
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
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Cited by 5 •
2019
Here we present a protocol for Ca2+ imaging in neurons and glial cells, which enables the dissection of Ca2+ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca2+ indicators.
FAQs
Natural tissue planes provide the paths where the amnion can be separated from surrounding placental tissues with less disruption. Following these planes helps maintain the membrane’s architecture and supports retention of its attached cellular layers. This preservation is important when the isolated tissue will be examined for extracellular matrix organization, cell biology, or tissue repair-related properties.
Blunt and sharp dissection provide controlled ways to separate the amnion while limiting unnecessary structural damage. Their use must support careful movement through the tissue interfaces rather than disrupting the membrane itself. Preserving the membrane’s structure and associated epithelial and mesenchymal cells improves its value for biological examination and subsequent processing.
An isolated membrane can support examination of extracellular matrix organization, epithelial cell biology, and mesenchymal cell biology. Because the tissue retains a layered biological structure, it also provides material for studying developmental processes and tissue repair. These features connect the dissection technique to questions about how placental tissues function and respond in biological settings.
A basic workflow prioritizes identifying the amnion within the fetal membranes, locating the separation interface, and progressing carefully through the surrounding placental tissues. Controlled blunt or sharp dissection is then used to isolate the membrane while preserving its structure and attached cellular layers. The resulting tissue can be directed toward examination or further processing.
Suitability depends on whether the isolated tissue retains the membrane’s structure and its attached cellular layers after separation. A preparation that preserves these features is better positioned for examining extracellular matrix organization, epithelial and mesenchymal biology, or repair-related properties. The quality of isolation therefore directly affects the biological information available from subsequent analysis.
The technique is useful when investigators need amniotic tissue to study barrier function, wound-healing properties, development, or potential regenerative applications. In clinical and research contexts, isolating the membrane creates material for examining how its structure and cellular components relate to tissue repair. It therefore links placental biology with investigations of healing and regeneration.