Dissection of Local Ca<sup>2+</sup> Signals in Cultured Cells by Membrane-targeted Ca<sup>2+</sup> Indicators

Hiroko Bannai; Matsumi Hirose; Fumihiro Niwa; Katsuhiko Mikoshiba

doi:10.3791/59246

Journal

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Neuroscience

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Dissection of Local Ca²⁺ Signals in Cultured Cells by Membrane-targeted Ca²⁺ Indicators

Dissection of Local Ca<sup>2+</sup> Signals in Cultured Cells by Membrane-targeted Ca<sup>2+</sup> Indicators

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Dissection of Local Ca²⁺ Signals in Cultured Cells by Membrane-targeted Ca²⁺ Indicators

9,017 Views

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11:33 min

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March 22, 2019

DOI:

10.3791/59246-v

DOI:

10.3791/59246-v

•

11:33 min

March 22, 2019

•

8997 Views

Hiroko Bannai^1,2, Matsumi Hirose², Fumihiro Niwa^2,3, Katsuhiko Mikoshiba²

¹Japan Science and Technology Agency,PRESTO ²Laboratory for Developmental Neurobiology,RIKEN Center for Brain Science ³École Normale Supèrieure, Institut de Biologie de l’ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University

Summary

Here we present a protocol for Ca²⁺ imaging in neurons and glial cells, which enables the dissection of Ca²⁺ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca²⁺ indicators.