Anti-icam-2 Antibody
Anti-icam-2 Antibody - Related Videos
13:01
Anti-Nuclear Antibody Screening Using HEp-2 Cells
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Cited by 34 •
2014
Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.
04:57
Detecting Circulating Anti-Glycan Antibodies with a Printed Glycan Array
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2025
This video demonstrates printed glycan array technology for analyzing circulating anti-glycan antibodies in mouse serum. The antibodies in the serum interact with specific glycans on the chip, which is confirmed by immunostaining followed by fluorescence scanning.
04:27
Effect of the Anti C-fms Antibody on Osteoclastogenesis in Mouse Bone Marrow Cells In Vitro
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2025
In this video, we demonstrate the effect of different anti-c-fms antibody concentrations on osteoclastogenesis, which is the process of osteoclast formation from osteoclast progenitors.
06:34
An Opsono-Adherence Assay for Assessing the Opsonization of Encapsulated Bacteria by Anti-Capsule Antibodies
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2025
This video demonstrates an opsono-adherence assay for examining the interaction between opsonized bacteria and immune cells. Fluorescently labeled bacteria, treated with test serum containing anti-capsule antibodies, are incubated with macrophages. After staining the macrophages with fluorescence, the attached bacteria on the macrophage surface are observed under a microscope for visualization.
05:54
An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα
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2025
This video demonstrates a technique for assessing the efficacy of antibodies against tumor necrosis factor-alpha (TNFα), an inflammatory cytokine, through an in vitro bioanalytical approach. TNFα, when introduced to stressed cells, triggers apoptosis. However, binding to the antibodies confines TNFα, averting apoptosis. The degree of apoptosis is quantified using a bioluminescence assay.
FAQs
Its behavior depends on the epitope, the specific region of ICAM-2 recognized by the antibody, and on how the reagent was designed. Binding may simply label ICAM-2 for detection, or it may affect interactions with integrins such as LFA-1. This distinction determines whether the antibody is most suitable for identification or for adhesion-related experiments.
An antibody that binds an interaction-relevant epitope may interfere with ICAM-2 engagement by LFA-1, allowing researchers to examine the contribution of this interaction to cell adhesion. In contrast, a detection-focused antibody can reveal ICAM-2 distribution without necessarily being intended to alter adhesion. The experimental interpretation therefore depends on the antibody’s design and application.
ICAM-2 participates in leukocyte adhesion and immune-cell interactions, so detecting it helps connect cell-surface expression with processes involving immune-cell contact and movement. This is relevant to endothelial and hematopoietic biology, as well as inflammatory research. The antibody provides a way to investigate where ICAM-2 is present while examining its relationship to cellular interactions.
The reagent is particularly relevant in studies of endothelial cells, hematopoietic cells, and inflammatory processes. These settings allow researchers to examine ICAM-2 in vascular biology, immune regulation, and cell trafficking. Its value comes from linking molecular detection of the cell-surface protein with broader questions about how immune and vascular cells interact.
In flow cytometry, the antibody is used to identify cells that express ICAM-2 by detecting antibody-associated labeling on individual cells. This approach supports analysis of ICAM-2-positive populations within a biological sample. It is useful when researchers need to assess cell-surface expression while studying hematopoietic or immune-cell populations and their interactions.
Immunofluorescence and immunohistochemistry extend ICAM-2 detection into cellular or tissue contexts, where researchers can examine its presence in relation to biological structures. These methods support studies of endothelial, hematopoietic, and inflammatory processes. Compared with cell-population analysis, they help investigate where ICAM-2-expressing cells or signals occur within the examined material.
Cell-based adhesion studies can test how ICAM-2-related interactions contribute to cell attachment and immune-cell engagement. Depending on the antibody’s design, researchers may use it to detect ICAM-2 or to interfere with its interaction with LFA-1. The resulting observations help evaluate mechanisms relevant to leukocyte adhesion, cell trafficking, and vascular biology.