Arabidopsis Leaves

Arabidopsis leaves are the photosynthetic organs of Arabidopsis thaliana, a small flowering plant widely used to investigate plant biology because its leaves are accessible, genetically tractable, and responsive to environmental conditions. Within leaf cells, chloroplasts capture light energy and use it to convert carbon dioxide and water into sugars, while stomata regulate gas exchange and water loss by opening or closing in response to signals such as light and humidity. Researchers examine Arabidopsis leaves to study photosynthesis, plant development, immunity, and responses to drought, salinity, and pathogens. Their reproducible structure and extensive genetic resources make them valuable for linking genes to cellular and whole-plant traits.

Arabidopsis Leaves - Related Videos

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JoVE EoE - Bacterial Pathogenesis and Host Interactions

Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration

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2026

Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.

Assessing Pseudomonas syringae Infection Load in Arabidopsis thaliana Leaves

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2026

Source: Liu, X., et al. Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem. J. Vis. Exp. (2015).This video demonstrates a method to assess the bacterial load in Arabidopsis thaliana leaves infected with Pseudomonas syringae. Leaf discs from infected leaves are homogenized, serially diluted, and plated onto nutrient-rich agar to promote bacterial colony formation. Colony counts are then used to...

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JoVE Journal - Biology
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A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

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Cited by 30 •

2012

We describe the use of perfluorodecalin as an infiltrative mounting medium. This is a simple method for improving depth of imaging in Arabidopsis thaliana leaf tissue with minimal physiological impact.

Isolation and Purification of Plant Extracellular Vesicles from Arabidopsis Leaves Using an Optimized Apoplastic Wash Collection Method

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2026

This protocol describes an optimized method for isolating extracellular vesicles (EVs) from Arabidopsis thaliana leaves to support downstream molecular and functional analyses.

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JoVE Journal - Biology
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Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

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Cited by 21 •

2012

A method for producing Arabidopsis leaf protoplasts that are compatible with fluorescence activated cell sorting (FACS), allowing for studies of specific cell populations. This method is compatible with any Arabidopsis line that expresses GFP in a subset of cells.

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