Mammalian Ovulation
Mammalian Ovulation - Related Videos
05:41
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
0 Views •
Cited by 27 •
2017
This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.
03:20
Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation
0 Views •
2025
Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.
04:02
Embryo Transfer Into Rabbit: A Procedure for Laparoscopy-Guided Transfer of Embryos Into Oviduct of Ovulating Recipient Female Rabbit Model
0 Views •
2025
This video describes the technique of transferring embryos into the oviduct of a recipient female rabbit via Laparoscopy. It allows embryo remodeling in rabbits during its migration through the oviduct, eventually helping in successful implantation. This technique can also be used for stored embryos with good efficiency.
02:52
Expansion of Mutant Vaccinia Virus in Mammalian Cells
0 Views •
2026
Source: Yuan, M., et al. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (2016)This video demonstrates the expansion of plaque-derived recombinant virus in adherent mammalian cells. The virus carries a fluorescent reporter in place of a virulent gene, enabling infection tracking. Infected cells are harvested for PCR verification of the genetic modification.
08:39
Electroporation of Functional Bacterial Effectors into Mammalian Cells
0 Views •
Cited by 7 •
2015
Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
FAQs
The mid-cycle luteinizing hormone surge acts as the initiating endocrine signal. It promotes enzyme activity within the mature follicle, alters ovarian blood flow and pressure, and contributes to weakening and rupture of the follicle wall. These linked changes convert a hormonal signal into the physical release of the oocyte, illustrating how endocrine regulation produces a coordinated reproductive event.
Each factor contributes a different part of follicle rupture. Enzyme activity supports changes in follicular tissues, while altered ovarian blood flow and pressure help create the physical conditions associated with wall disruption. Their coordination matters because ovulation is not a single molecular event, but a sequence of follicular and ovarian changes that culminates in oocyte release.
After release, the oocyte is captured by the uterine tube, placing it within the reproductive tract where it may encounter conditions relevant to fertilization. This capture connects the ovarian event with events occurring elsewhere in the reproductive system. Consequently, successful ovulation depends not only on follicle rupture but also on coordinated changes in the reproductive tract.
Ovulation represents a coordinated point within menstrual and estrous cycles, linking ovarian activity with changes in the reproductive tract. Studying when and how the follicle responds to the luteinizing hormone surge helps explain how endocrine signals organize these cycles. The process therefore provides a biological framework for understanding recurring reproductive function across mammalian systems.
Because fertility depends on coordinated ovarian and reproductive-tract events, disruptions involving follicle maturation, hormone signaling, follicle rupture, or oocyte capture may affect reproductive potential. Studying mammalian ovulation helps researchers understand infertility and informs assisted reproduction. The process is therefore relevant both for identifying where reproductive coordination may fail and for developing approaches that address such failures.
Ovulation demonstrates how an endocrine signal can coordinate events across tissues. The luteinizing hormone surge affects the follicle through enzyme activity, blood-flow and pressure changes, while the reproductive tract participates by capturing the released oocyte. This integrated response provides insight into hormonal regulation of reproduction and helps explain the biological basis of hormonal contraception.