Cardiomyocyte Ablation
Cardiomyocyte Ablation - Related Videos
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
08:03
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Cited by 25 •
2016
Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.
11:21
Robotic Ablation of Atrial Fibrillation
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Cited by 12 •
2015
Pulmonary vein isolation (PVI) with an ablation catheter is a curative treatment for atrial fibrillation (AF). Robotic catheter systems aim to improve catheter steerability. Here, a procedure with a new robotic catheter system is presented. The goal of the procedure is electrical block between pulmonary vein and left atrium.
04:42
Targeted Neuronal Ablation Using Two-Photon Microscopy in a Zebrafish Larava
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2025
Source: Muto, A., et al. Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae. J. Vis. Exp. (2018) In this video, a two-photon laser ablation procedure is demonstrated, enabling the selective destruction of fluorescent neurons in agarose-immobilized zebrafish larvae. High-resolution imaging before and after the procedure confirms efficient neuronal damage, as observed by the absence of fluorescence.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
FAQs
Selective damage helps researchers attribute later changes to the loss of contractile heart muscle cells rather than to nonspecific injury alone. This makes it possible to examine how the heart responds through cell proliferation, inflammatory signaling, scar formation, and altered cardiac function. The resulting model connects a defined cellular event with tissue-level repair or remodeling outcomes.
The model allows investigators to compare replacement of damaged cardiomyocytes with responses that preserve injury through scarring or functional decline. Increased cell proliferation may indicate a repair response, whereas scar formation and worsening cardiac function are consistent with pathological remodeling. Monitoring these outcomes together helps determine whether the heart is restoring tissue or adapting to persistent damage.
Cell proliferation, inflammatory signaling, scar formation, and cardiac function provide complementary measures of the injury response. Proliferation addresses whether cells enter a repair-associated state, inflammation reflects signaling after damage, and scarring indicates structural remodeling. Functional measurements show whether cellular and molecular changes translate into improved or impaired heart performance.
Researchers can induce the injury through genetic, chemical, or physical approaches, selecting the strategy that best suits the biological question. Regardless of the approach, the study begins with targeted cardiomyocyte damage and then follows the heart’s cellular and molecular responses. This common experimental logic supports comparisons among injury conditions while retaining a defined starting event.
A typical workflow establishes targeted cardiomyocyte injury, then monitors the heart for cellular, molecular, and functional consequences. Investigators can assess proliferation, inflammatory signaling, scar formation, and cardiac performance as the response develops. Comparing these measurements after ablation helps relate the initial injury to repair capacity, tissue remodeling, and overall cardiac outcome.
Cardiomyocyte ablation is useful when researchers need a controlled way to test whether particular factors support repair after cardiac injury. By examining regeneration-associated responses alongside scarring and functional changes, investigators can evaluate candidate influences on heart recovery. In biology, the approach also helps clarify why some injury responses may restore tissue whereas others lead to pathological remodeling.