Axon Sealing
Axon Sealing - Related Videos
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
03:24
Two-Photon Laser Axotomy: A Method to Injure Axons in Zebrafish Embryos and Observe Axonal Recovery
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2023
This video describes the method to injure axons in zebrafish embryos using two photon laser axotomy and observing axonal recovery from injury.
07:51
The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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Cited by 8 •
2010
The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
09:25
Laser Nanosurgery of Cerebellar Axons In Vivo
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Cited by 7 •
2014
Two-photon imaging, coupled to laser nanodissection, are useful tools to study degenerative and regenerative processes in the central nervous system with subcellular resolution. This protocol shows how to label, image, and dissect single climbing fibers in the cerebellar cortex in vivo.
03:57
Visualization of Axonal Projection Patterns of Embryonic Motor Neurons in Drosophila
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2025
Source: Jeong, S. Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila. J. Vis. Exp. (2017)This video demonstrates the visualization of axonal projections in Drosophila embryos. Immunolabeling of motor neurons, precise dissection to expose neuronal structures, and differential interference contrast microscopy collectively reveal detailed neuronal architecture, emphasizing the axonal projection patterns.
FAQs
Calcium entry acts as the immediate signal that mobilizes repair at an axonal lesion. When the membrane is disrupted, calcium ions enter the axon and promote the accumulation and fusion of intracellular vesicles at the damaged site. This targeted vesicle response helps close the opening and limits continued loss of the neuron's internal contents.
Vesicle fusion does not complete the repair response. Cytoskeletal processes and additional membrane-repair activity help organize and stabilize the newly formed seal. This stabilization is important because a closure must become sufficiently secure to preserve axonal integrity. In experimental biology, examining these supporting processes helps distinguish initial closure from successful membrane repair.
A successful repair response supports neuronal survival by protecting the internal environment after injury. It also affects what happens next: the damaged axon may retain the capacity to regrow or reconnect with its target. Thus, sealing is not only an immediate membrane event; it can influence longer-term structural recovery and the restoration of connections.
Experimental studies can focus on the sequence of events at the lesion: calcium entry, intracellular-vesicle accumulation and fusion, and stabilization by cytoskeletal and membrane-repair processes. Researchers can also manipulate or assess axonal repair, using sealing as one feature of the response. This framework connects cellular mechanisms with survival and later regrowth or reconnection.
It is especially relevant in research on nerve injury, neurodegeneration, and neural regeneration. In nerve-injury studies, sealing addresses the earliest membrane damage. In degeneration research, it provides a way to consider how insufficient repair may relate to neuronal loss. In regeneration studies, it helps connect membrane repair with the possibility of axonal regrowth or target reconnection.
Assessment can reveal whether the damaged membrane has undergone the coordinated repair response described for axons: calcium-triggered vesicle recruitment and fusion followed by stabilization of the seal. Interpreting these features helps researchers evaluate repair at the lesion and relate it to broader outcomes, including neuronal survival and the axon's potential to regrow or reconnect with its target.