Bacterial Samples
Bacterial Samples - Related Videos
09:03
Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample
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2023
Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Seemingly impossible to determine, microbial biodiversity is truly astounding with an estimated one trillion coexisting species (1,2). Although particularly harsh climates, like the acidic environment of the human stomach (3) or the subglacial lakes of Antarctica (4), may be dominated by a specific species, bacteria are typically...
10:44
Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
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Cited by 1 •
2025
Here, we present a protocol to enable whole-genome sequencing of bacterial sexually transmitted infections from clinical samples using target enrichment. This novel, syndromic panel-based method overcomes challenges of abundant human DNA and low bacterial loads, facilitating genomic surveillance for Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, and Mycoplasma genitalium.
03:57
Bacterial Infection Quantitation Assay: A Method to Quantify Intraocular Bacterial Load in Mouse Model of Bacterial Endophthalmitis
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2025
This video describes the technique of quantifying bacterial infection from eye homogenates obtained from a murine model of bacterial endophthalmitis caused by Bacillus cereus. This technique helps study the host immune response and its dependency on the host and ocular bacterial gene expression in murine models.
05:21
Bacterial Transformation - Student Protocol
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2019
Bacterial Transformation ExpandIMPORTANT: In addition to wearing the appropriate personal protective equipment, be sure to take care to keep your face away from suspension cultures, and to avoid inhaling reagents. Do not touch your face while performing the experiment. Always wash your hands before and after every experiment. When you are ready to safely begin, check to make sure you have a tube or tubes of competent E. coli cells in your ice bucket. Then, transfer 50 µL from one of these tubes...
14:05
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
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Cited by 7 •
2013
Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host cells.
FAQs
Representative sampling helps ensure that the material analyzed reflects the bacterial population or condition being investigated. A poorly selected sample may overemphasize certain cells or miss relevant microbial diversity, reducing the reliability of later observations. In biology, careful sampling strengthens conclusions about environmental change, disease, food safety, and microbial function by connecting laboratory findings to the original source.
Aseptic handling limits the introduction of unwanted microorganisms, while suitable transport and preservation help maintain the sample’s relevant condition during processing. These steps are especially important when analysis depends on viable cells or comparisons among samples. If handling allows contamination or changes that affect viability, subsequent culture, detection, microscopy, biochemical testing, or genomic analysis may no longer represent the original material.
Culture-based approaches support identification by allowing bacterial cells to be examined through growth and subsequent testing. Other detection approaches can contribute information without relying exclusively on the same growth process, while microscopy, biochemical testing, and genomic analysis provide additional perspectives. Using complementary methods can improve interpretation of bacterial presence, characteristics, diversity, or function rather than relying on one result alone.
A useful workflow begins with representative collection, followed by aseptic handling and transport or preservation appropriate to the intended analysis. The material can then undergo growth or detection, with microscopy, biochemical testing, culture-based identification, or genomic analysis selected according to the research question. Maintaining sample quality throughout these stages improves the consistency and interpretability of the resulting data.
Bacterial samples support investigations across environmental, clinical, food, and laboratory settings. They can help researchers examine disease, monitor environmental change, evaluate food safety, study microbial diversity and function, and investigate antimicrobial responses. The same general principles of careful collection and preparation allow bacterial evidence to be applied to both descriptive studies and questions involving biological effects or biotechnology.
Analysis can provide evidence for identifying bacteria, observing cells, evaluating biochemical characteristics, examining genomic information, or describing microbial diversity and function. The outcome depends on the selected preparation and analytical method, as well as the quality of the original material. In biology, these results can support conclusions about disease, environmental conditions, food safety, antimicrobial responses, or biotechnology applications.