Bmdm Cells
Bmdm Cells - Related Videos
10:42
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
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Cited by 11 •
2016
Here we describe a method for bacterial RNA isolation from Listeria monocytogenes bacteria growing inside murine macrophages. This technique can be used with other intracellular pathogens and mammalian host cells.
03:41
Isolation of Intracellular Pathogenic Bacteria from Infected Host Cells
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2026
Source: Sigal, N., et al. RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells. J. Vis. Exp. (2016).This video demonstrates the isolation of internalized pathogenic bacteria from infected bone marrow-derived macrophages (BMDMs). Bacteria are allowed to attach to host cells, and unattached bacteria are washed away. After internalization, an antibiotic is added to eliminate non-internalized bacteria. The host cells are lysed, centrifuged, and the supernatant is...
10:07
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Cited by 326 •
2013
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
08:34
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
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Cited by 13 •
2017
Here, we describe the purification of Legionella pneumophila (L. pneumophila) outer membrane vesicles (OMVs) from liquid cultures. These purified vesicles are then used for the treatment of macrophages to analyze their pro-inflammatory potential.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
FAQs
Macrophage colony-stimulating factor (M-CSF) provides the culture signal that directs bone marrow progenitors toward a macrophage state. The resulting BMDM population can then be exposed to defined environmental signals, allowing investigators to examine how developmental conditioning influences later immune behavior under controlled laboratory conditions.
Pattern-recognition receptors (PRRs) act as sensing components that connect environmental cues with macrophage responses. When BMDMs encounter relevant signals, PRR activity can be evaluated through changes in cytokine production, phagocytosis, and gene expression. This makes the cells useful for dissecting how innate immune detection is translated into distinct cellular outputs.
Changes in cytokine production, phagocytosis, and gene expression capture different aspects of the response to an environmental signal. Examining them together allows investigators to determine whether a condition affects immune mediators, macrophage function, gene activity, or several features at once. The combined profile supports more precise interpretation of experimental outcomes.
Controlled culture conditions make it possible to relate a defined environmental change to a macrophage outcome. In BMDMs, investigators can examine whether the same change alters cytokine production, phagocytosis, gene expression, or multiple responses together. This control supports mechanistic studies by connecting an experimental condition with a measurable cellular effect.
Researchers begin with bone marrow cells, culture the progenitors with M-CSF, and allow differentiation toward macrophages. Once the cells have developed, experiments can expose them to environmental signals and assess cytokine production, phagocytosis, or gene expression. This workflow links a controlled culture stage to measurable immune-cell responses.
BMDM cells support studies across innate immunity, inflammation, host-pathogen interactions, and tissue repair. They are also useful for testing responses to drugs or genetic perturbations. Across these applications, the culture system lets researchers examine macrophage behavior in a controlled setting while focusing on cellular changes relevant to a particular biological question.
Researchers can apply a drug or genetic perturbation to BMDMs and examine resulting changes in cytokine production, phagocytosis, or gene expression. These comparisons help identify how an intervention affects macrophage biology and can connect a cellular mechanism with an outcome relevant to disease-focused research questions.