Bag Cell Culture
Bag Cell Culture - Related Videos
09:32
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
0 Views •
Cited by 98 •
2014
We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.
08:05
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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Cited by 3 •
2016
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
08:32
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
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Cited by 133 •
2009
Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.
16:29
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
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Cited by 18 •
2008
Aplysia californica neurons develop large growth cones in culture that are excellent for high-resolution imaging of growth cone motility and guidance. Here, we present a protocol for dissection and plating of Aplysia bag cell neurons as well as for setting up a chamber for live cell imaging.
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
FAQs
Gas-permeable bag materials allow oxygen transfer while the rocking or mixing system distributes nutrients throughout the medium. This movement also helps prevent local depletion and supports waste removal from the cell environment. Together, these features maintain more consistent culture conditions than relying on a static fluid volume, which is important when expanding cells under controlled laboratory or bioprocessing conditions.
A closed bag limits repeated exposure of the culture to the surrounding environment during handling. This can reduce contamination risk and simplify routine operations compared with workflows that require frequent opening of a rigid vessel. The single-use format also reduces the need to prepare the same vessel for repeated use, supporting consistent handling across experiments or production activities.
Cell expansion depends on maintaining suitable access to nutrients, oxygen transfer, and removal of metabolic waste. The amount of medium, the cell inoculum, and the effectiveness of rocking or mixing all contribute to how evenly these conditions are maintained. Controlling these factors helps provide a stable environment for mammalian, immune, and other biological cell cultures.
A typical setup places nutrient medium and a defined cell inoculum into a sterile, flexible bag. The bag is then used with a rocking or mixing system so nutrients and gases can be distributed while waste is removed. Maintaining the closed configuration during culture reduces environmental exposure and supports controlled growth through the desired expansion period.
Researchers may select this approach when they need controlled expansion with reduced contamination risk, simplified handling, or a transition from small experiments to larger systems. It is relevant to mammalian and immune-cell culture, as well as vaccine production, protein manufacturing, and cell-based therapies. The choice is especially useful when a closed, single-use workflow fits the study or process.
Bag Cell Culture can support the expansion of cells needed for biological research and for manufacturing-related activities. Depending on the cell type and purpose, the culture may contribute to vaccine production, protein manufacturing, or cell-based therapies. Its value lies in providing a controllable environment that can be adapted from exploratory work to larger-scale production.