Carbon Nanotube Lysis
Carbon Nanotube Lysis - Related Videos
10:26
Transport of Surface-modified Carbon Nanotubes through a Soil Column
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Cited by 5 •
2015
Surface properties of a nanoparticle are important for their interaction with the surrounding medium. Therefore the surface modification of carbon nanotubes can be critical for their transport and retention through porous media. Here, lab scale column experiments are used to understand the possible transport and retention of these nanoparticles.
09:23
Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures
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Cited by 7 •
2012
We present methods for fabrication of patterned microstructures of vertically aligned carbon nanotubes (CNTs), and their use as master molds for production of polymer microstructures with organized nanoscale surface texture. The CNT forests are densified by condensation of solvent onto the substrate, which significantly increases their packing density and enables self-directed formation of 3D shapes.
11:09
Grafting Multiwalled Carbon Nanotubes with Polystyrene to Enable Self-Assembly and Anisotropic Patchiness
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Cited by 5 •
2018
A procedure for the synthesis of polystyrene-grafted multiwalled carbon nanotubes using successive chemical modification steps to selectively introduce the polymer chains to the sidewalls and their self-assembly via anisotropic patchiness is presented.
08:10
Precision Milling of Carbon Nanotube Forests Using Low Pressure Scanning Electron Microscopy
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2017
Low pressure scanning electron microscopy in a water vapor ambient is used to machine nanoscale to microscale features in carbon nanotube forests.
14:09
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
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Cited by 18 •
2013
In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.
FAQs
Carbon nanotubes can disrupt cell envelopes through contact between their nanoscale surfaces and the surrounding membrane structure. This damage can make intracellular material accessible for collection. The extent of disruption is not fixed by nanotube presence alone; it depends on the nanotube’s characteristics and the biological cell being treated.
Externally applied ultrasound or light can increase disruption beyond nanotube and membrane contact alone. Ultrasound contributes mechanical effects, whereas light can produce photothermal effects through energy absorbed by the nanotubes. These enhancements may support faster or more effective release, but their outcome depends on the operating conditions used during sample preparation.
Efficiency depends on several interacting variables, including nanotube type, surface chemistry, cell structure, and operating conditions. These factors influence how effectively the nanotubes interact with cell envelopes and how much externally applied energy contributes to disruption. Considering them together is important when adapting the approach to different biological samples or analytical goals.
A general workflow begins by bringing the selected nanotubes into contact with the cells, then applying ultrasound or light when additional mechanical or photothermal disruption is desired. After the cell envelope is damaged, the released intracellular material is recovered for downstream assays. The sequence connects nanoscale treatment with subsequent biological analysis.
The approach can provide access to nucleic acids, proteins, and other cellular contents that were previously enclosed within cells. Recovering these materials supports downstream assays, although the specific material obtained and its usefulness depend on how completely the cell envelope is disrupted and how the sample is handled afterward.
Carbon Nanotube Lysis links nanomaterials engineering with biological sample preparation. Its potential value comes from combining nanoscale membrane interaction with optional ultrasound or light enhancement, which may enable rapid lysis and analysis. In biology, this creates a route for preparing cellular contents for downstream investigation while highlighting the importance of cell structure and operating conditions.