Bloodmeal Screening
Bloodmeal Screening - Related Videos
09:05
Meal Duration as a Measure of Orofacial Nociceptive Responses in Rodents
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Cited by 3 •
2014
A lengthening in meal duration represents orofacial nociceptive behavior in rodents similar to the guarding behavior of humans with orofacial pain. Eating is a behavior that requires no training or animal manipulation, requires cortical participation, and is not competing with other experimentally induced behaviors, distinguishing this assay from alternative reflex or operant measurements.
08:09
Preparation and Testing of Plant Seed Meal-based Wood Adhesives
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Cited by 15 •
2015
To facilitate the effort in seeking more economic and environment-friendly formulations of natural product-based wood adhesives, this work demonstrates the preparation and testing of plant seed-based wood adhesives. This protocol allows one to assess plant seed-based agricultural products as suitable candidates for the substitution of synthetic-based wood adhesives.
09:42
Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes
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Cited by 17 •
2020
The goal of this protocol is to deliver animal-derived and artificial blood meals to Aedes aegypti mosquitoes through an artificial membrane feeder and precisely quantify the volume of meal ingested.
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
FAQs
After DNA is extracted from a vector’s abdomen, polymerase chain reaction assays, sequencing, or species-specific genetic markers generate host-related genetic information. Researchers compare these signatures with reference databases, allowing the consumed blood source to be assigned to an animal or human host when a matching genetic profile is available.
A genetic signature becomes useful through comparison with known reference signatures. These databases help researchers connect DNA recovered from an abdomen to a particular animal or human host. The quality and coverage of available references therefore influence how confidently feeding patterns, host preferences, and vector contact with reservoir hosts can be interpreted.
PCR assays, sequencing, and species-specific genetic markers provide alternative ways to examine host DNA recovered from a vector. Each approach produces information that can be compared with reference databases. These molecular tools enable researchers to move from extracted DNA to an identified host and then to ecological interpretation.
The workflow begins by obtaining a blood-fed mosquito or tick and focusing on its abdomen, where host DNA is recovered. Researchers extract that DNA, apply PCR, sequencing, or species-specific marker assays, and compare the resulting genetic signature with reference databases to identify the likely animal or human host.
By identifying hosts across examined mosquitoes or ticks, researchers can determine which animals or humans those vectors have fed on. Aggregated identifications reveal feeding patterns and host preferences, while detections involving reservoir hosts show where vectors and those hosts come into contact, providing ecological context for transmission studies.
Host identities recovered from blood meals help researchers evaluate whether vectors contact reservoir hosts and assess potential transmission risk. In biology, these results support vector ecology studies, disease-transmission investigations, and surveillance. They can also inform vector control strategies by indicating which host associations deserve attention.