Bone Wax Occlusion

Bone wax occlusion is a surgical method used to control bleeding from exposed cancellous bone by sealing open marrow spaces. It works through mechanical compression and physical blockage: wax is pressed into the cut bone surface, where it reduces blood flow from small vessels and creates a temporary barrier. In biology and biomedical research, this principle supports hemostasis during procedures involving bone cutting or drilling, helping maintain a clearer operative field and illustrating how material-based occlusion can alter tissue fluid movement.

Bone Wax Occlusion - Related Videos

Research

JoVE EoE - Large Animal Models

Generating Rabbit Model of Bone Infection: A Technique of Inducing Chronic Infection by Injecting Bacteria Directly in Bone Marrow

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2025

This video describes the technique of surgically inducing bacterial infection in the bone marrow of a rabbit by using bone wax to avoid the leakage of bacteria. This rabbit model can be used to study bone infection treatment and bone regeneration.

Models of Bone Metastasis

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Cited by 115 •

2012

Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.

Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration

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2023

In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.

Research

JoVE Journal - Immunology and Infection
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Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

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Cited by 293 •

2016

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation

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2023

This video describes the culturing of breast cancer cells adjacent to femur bone explants to measure cancer cell proliferation and colonization in a human bone tissue explant model system.

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