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JoVE Journal
Immunology and Infection

This content is Open Access.

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
 

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Article DOI: 10.3791/53936
April 14th, 2016

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Summary April 14th, 2016

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

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