Carbon 13 Spectroscopy
Carbon 13 Spectroscopy - Related Videos
01:10
Carbon-13 (¹³C) NMR: Overview
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2024
Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
11:43
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Cited by 7 •
2016
Dynamic nuclear polarization with subsequent sample dissolution has enabled real-time studies of metabolism in biological systems. Hyperpolarized [1-13C]pyruvate was used to study lactate dehydrogenase activity in a prostate carcinoma cell line in vitro.
![Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-<sup>13</sup>C]Pyruvate and <sup>13</sup>C/<sup>31</sup>P NMR Spectroscopy](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63188.jpg%3Fv%3D2026-09-01%252019%253A57%253A02&w=750&q=75)
14:56
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
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2023
We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.
13:59
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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Cited by 4 •
2013
Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
FAQs
Chemical shifts in carbon-13 spectroscopy indicate the chemical environment around each carbon-13 nucleus. Differences arise from molecular structure, bonding, and nearby atoms, so signals can distinguish chemically different carbon sites. Interpreting these shifts allows researchers to connect spectral features with structural changes in carbon-containing molecules.
Carbon-13 labeling provides a traceable route through complex biological reactions. When labeled nutrients move through metabolic pathways, spectroscopy can help follow their carbon and identify the metabolites produced along the way. This makes it possible to examine how carbon is transformed rather than simply observing the final molecular mixture.
Signal patterns can reveal changes associated with metabolism, enzyme activity, and biosynthesis. Because the signals reflect the chemical environments of carbon atoms, researchers can examine how enzymatic reactions alter carbon-containing molecules and how those molecules are assembled or transformed. The same approach also supports analysis of cellular carbon flux through pathways.
The analysis applies a magnetic field and radiofrequency pulses to produce signals from carbon-13 nuclei. Researchers then examine the resulting signal differences, especially chemical shifts, to assess molecular structure, bonding, and neighboring atoms. When biological samples contain labeled nutrients, the observed signals can also be interpreted to track transformations through metabolic pathways.
Researchers can use carbon-13 spectroscopy when they need to trace nutrients through metabolism, identify metabolites, or examine biomolecular structure. It is especially relevant to studies of enzyme activity, biosynthesis, and cellular carbon flux, where following labeled carbon provides information about reactions and pathway transformations that may not be evident from endpoint measurements alone.
Metabolic studies can use the technique to identify metabolites and follow carbon movement through complex reaction pathways. The resulting information helps connect labeled nutrients with downstream molecular products and supports examination of cellular carbon flux. It can also show how enzymatic activity and biosynthetic processes contribute to changes in carbon-containing molecules.