Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Chemistry

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
 

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Article doi: 10.3791/50875
December 12th, 2013

Summary December 12th, 2013

Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter