Cardiac Perfusion Procedure
Cardiac Perfusion Procedure - Related Videos
11:30
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
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Cited by 68 •
2012
A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.
06:22
Modified Langendorff Perfusion for Extended Perfusion Times of Rodent Cardiac Grafts
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Cited by 2 •
2024
This article demonstrates the feasibility of achieving longer perfusion times (4 h) of murine cardiac grafts without function loss by employing lower (30-35 mmHg) than physiological (60-80 mmHg) perfusion pressures during Langendorff.
07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Cited by 93 •
2013
A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.
06:02
Anatomical Reconstructions of the Human Cardiac Venous System using Contrast-computed Tomography of Perfusion-fixed Specimens
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Cited by 9 •
2013
The objective of this research is to recreate and then access the anatomy of the human cardiac venous system using 3D reconstructions generated from contrast-computed tomography scans.
09:38
Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
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Cited by 18 •
2015
The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.
FAQs
The perfusate reaches the coronary circulation through the aorta, so researchers can regulate delivery by controlling pressure or flow. This makes it possible to examine how coronary resistance and cardiac performance change under defined circulation conditions. Separating these variables helps distinguish effects caused by perfusion itself from changes associated with the heart’s workload or experimental treatment.
Oxygen and nutrients help sustain cardiac tissue during the experiment, while the perfusate also provides a route for removing metabolic waste. Temperature and solution composition can be adjusted to create different experimental conditions. Controlling these factors is essential because changes in tissue support may alter contractility, heart rate, electrical activity, or other measured indicators of function.
These experiments can quantify contractility, heart rate, coronary resistance, and electrical activity in an isolated preparation. Together, these measurements describe mechanical performance, vascular behavior, rhythm, and cardiac excitation. Because the preparation is separated from influences of the intact animal, researchers can relate changes in these outputs more directly to perfusion conditions, workload, metabolism, or an applied treatment.
An isolated heart or cardiac tissue preparation removes influences arising from the intact animal while retaining measurable cardiac function. Researchers can therefore adjust perfusate composition, temperature, pressure or flow, and workload independently. This controlled setting supports mechanistic biology experiments, although the measurements describe the isolated preparation rather than the complete cardiovascular system.
A typical setup begins with an isolated heart or cardiac tissue and an oxygenated, nutrient-rich perfusate. The solution is directed through the aorta into the coronary circulation, with pressure or flow maintained at the selected condition. Researchers then adjust temperature, composition, or workload as needed and record functional outcomes such as contractility, heart rate, resistance, and electrical activity.
The method is useful when investigators need to examine cardiac physiology under controlled conditions or test how a defined change affects function. Applications include studies of ischemia-reperfusion injury, drug effects, metabolism, and mechanisms of cardiac disease. Measurements from the isolated preparation can show how these conditions influence contractility, coronary resistance, heart rate, or electrical activity.
A cardiac perfusion procedure allows researchers to manipulate the conditions supplying the isolated heart or tissue and then assess functional consequences through measurable cardiac outputs. In ischemia-reperfusion research, the preparation provides a controlled system for examining changes associated with interrupted and restored support. Contractility, coronary resistance, heart rate, and electrical activity can reveal different aspects of the response.