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Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
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Bioengineering
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JoVE Journal Bioengineering
Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

DOI:
10.3791/53029-v

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09:38 min

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August 10, 2015

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, , , , , , , , , ,
1General, Visceral, and Transplantation Surgery,Charité – Universitätsmedizin Berlin

Chapters

  • 00:05Title
  • 01:44Pre-surgical Preparation
  • 02:40Liver Harvest
  • 06:18Decellularization
  • 07:14Results: Representative Liver Decellularization
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.

Tags

Keywords: DecellularizationRat LiverOscillating PressurePerfusion DeviceExtracellular MatrixRecellularizationPortal VeinHepatic ArteryCorrosion CastingHistologyDNA Content Analysis

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