Carotid Artery Ligation
Carotid Artery Ligation - Related Videos
11:00
A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium
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Cited by 83 •
2010
This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.
04:28
Cannulation of the Carotid Artery in a Rat Model
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2025
In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.
10:32
Mechanical Testing of Mouse Carotid Arteries: from Newborn to Adult
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Cited by 31 •
2012
Passive mechanical testing of mouse carotid arteries is described, with special consideration for adapting to different specimen ages. The procedures include determining the in vivo length of the artery, mounting it in a pressure myograph, recording data, measuring the unloaded dimensions and analyzing the resulting data.
03:42
Modeling Brain Tumor Metastasis via Internal Carotid Artery Injection of Cancer Cells
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2025
Source: Lim, M., et al. Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells. J. Vis. Exp. (2022)In this video, an anesthetized mouse undergoes a surgical procedure in which cancer cells are injected into the common carotid artery (CCA) to facilitate targeted delivery to the brain. The procedure involves precise suturing and manipulation of the carotid arteries to direct the injected cells toward the internal carotid artery (ICA), promoting tumor formation in the brain...
04:33
Carotid Artery and Jugular Vein Interposition: A Technique to Induce Venous Intimal Hyperplasia in Rabbit Model
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2025
This video demonstrates the interposition of the rabbit carotid artery with the jugular vein to induce venous intimal hyperplasia by subjecting the veins to arterial pressure. This model will help investigate molecular and genetic interventions to attenuate the progression of intimal hyperplasia after revascularization surgeries.
FAQs
The intervention reduces perfusion through the treated carotid artery, which can lower oxygen delivery and alter pressure in regions supplied by that vessel. These changes create a controlled vascular challenge rather than an isolated structural lesion. Researchers can then examine how reduced blood supply affects cerebral function, tissue condition, and subsequent adaptation.
Collateral circulation determines how effectively other vessels compensate for the reduced flow. Stronger compensation may limit the severity of cerebral perfusion changes, whereas limited compensation can increase oxygen deficiency and vascular stress. Measuring outcomes alongside blood-flow changes therefore helps researchers distinguish the direct effect of occlusion from the tissue response to inadequate compensation.
The duration of occlusion changes the biological question being tested. A temporary restriction can reveal responses during reduced blood supply and after flow is restored, while a permanent ligation supports studies of sustained vascular insufficiency. Comparing these conditions helps identify effects associated with persistent ischemic stress versus tissue adaptation over time.
In an animal research procedure, the carotid artery is exposed and constricted with a suture or another ligature. The researcher establishes either a temporary or permanent reduction in flow, depending on the experimental design, and then evaluates the resulting vascular and biological changes. The procedure must be linked to measurements that document the physiological consequence of occlusion.
Behavioral observations, blood-flow measurements, and pathological assessment provide complementary evidence. Behavior can indicate functional consequences, flow measurements describe the vascular change, and pathology reveals tissue injury or structural responses. Considering these outcomes together allows researchers to connect altered perfusion with cellular damage, neurovascular responses, and signs of recovery.
Carotid artery ligation provides an animal-model approach for examining how reduced cerebral blood supply affects the nervous system. It supports investigations of ischemia, stroke-related injury, neurovascular responses, and tissue adaptation. Because researchers can relate vascular changes to behavior and pathology, the method helps connect altered circulation with functional and cellular outcomes.