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Chapters
- 00:00Title
- 00:42Introduction
- 01:24Partial Ligation of the Left Carotid Artery
- 04:21Carotid Ultrasound Examination to Validate Disturbed Carotid Flow
- 06:28Intimal RNA Isolation From Carotid
- 09:19Representative Results
- 10:11Conclusion
This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.
Tags
Disturbed Flow-induced AtherosclerosisMouse Carotid ArteryPartial LigationRNA IsolationEndotheliumAtherogenesisLow Flow ConditionsOscillatory FlowCommon Carotid ArteryDirect EvidenceRapid Atherosclerosis DevelopmentApolipoprotein E Knockout MouseEndothelial DysfunctionHyperlipidemic Mouse ModelComplex Lesion FormationIntraplaque NeovascularizationMolecular MechanismsFlow-dependent RegulationVascular BiologyTherapeutic Interventions
