Cell Culture
Cell Culture - Related Videos
08:05
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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Cited by 3 •
2016
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
07:01
Induction and Testing of Hypoxia in Cell Culture
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Cited by 247 •
2011
Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.
06:54
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
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Cited by 12 •
2012
Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.
02:47
Human ES cells: Starting Culture from Frozen Cells
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2006
Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.
01:21
Cell Culture
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2023
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media Culture conditions The growth medium is a crucial...
FAQs
These conditions work together to keep cells viable and behaviorally consistent outside the organism. Temperature, pH, and gas levels create the environment needed for cellular maintenance, while sterile technique limits unwanted contamination that could alter the culture. Controlling these variables allows researchers to examine cellular responses under defined conditions rather than under changing laboratory influences.
Growth format determines how cells are physically maintained in the laboratory. Adherent cells attach to a treated surface, whereas suspension cells remain distributed throughout the nutrient-containing medium. This distinction affects how researchers organize the culture and expand cells, and it provides a way to match the laboratory environment to the growth behavior of the cells being studied.
Expansion increases the available cell population when researchers need more material for study, while differentiation directs cultures toward specialized cellular states when changes in cell identity or function are important. Because both processes occur under controlled conditions, investigators can use the same general culture system to support cell growth, developmental studies, or analyses of specialized cellular behavior.
A basic workflow places cells in nutrient-containing medium, establishes the appropriate temperature, pH, and gas levels, and applies sterile technique throughout maintenance. Researchers then retain the culture in the growth format appropriate to the cells, either attached to a treated surface or distributed in suspension. Depending on the study, the culture may subsequently be expanded or differentiated.
Cell Culture is useful when investigators need to examine cell signaling, development, disease mechanisms, or responses to drugs and environmental conditions under defined laboratory circumstances. The approach separates cellular behavior from the full complexity of an organism, helping researchers focus on how selected conditions influence cells. These experiments can therefore connect controlled environmental changes with specific cellular responses.
Cultured cells can be expanded or differentiated to provide controlled cellular material for tissue engineering and regenerative research. These capabilities help investigators study how cells behave before considering their use in larger biological systems. Cell culture also supports production of biological materials, extending its role beyond observation to the preparation of cells or cell-derived products for research applications.